FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130538143
Sequences flagged as poor quality0
Sequence length126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG45277593.468533331288465Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18670761.4302915278946475No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC15197021.164182334047758Illumina Single End PCR Primer 1 (100% over 50bp)
CGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAA2965550.22717881010456842No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2584920.19802028285326534No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG1904690.14591060943773346No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1681810.12883667266509222No Hit
GGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGA1498480.11479250168282232No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)