FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171084716
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC120036327.016191908107092TruSeq Adapter, Index 8 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30624741.7900336579452252No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG5085840.29727027164717623TruSeq Adapter, Index 8 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1857570.10857603434312624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)