FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182364578
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC4662620.25567574860946957No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT4414200.24205358564753734No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2124980.11652372534758366No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC2103220.11533051116977334No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2093380.11479093269966056No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2077100.1138982154747179Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC2059790.11294901798308661No Hit
CCCTAATAAAATTAATTAACCACTCATTCATCGACCTCCCCACCCCATCC2037890.11174812687582344No Hit
CAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGG2037760.11174099829847439No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT2034910.11158471794889904No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)