FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80148721
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT2845730.3550561960932602TruSeq Adapter, Index 27 (97% over 44bp)
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT1477930.18439845097465746No Hit
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTAT1442460.17997292807704318No Hit
TTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTC1045690.13046870704274868No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1035240.12916488087189815No Hit
CTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTT802080.10007396125510226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)