FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89428013
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC24780692.7710209775095866No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA14692761.6429706427671604No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC9963611.1141486504905347No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC8477590.9479792422537667No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC6769350.7569607970603126No Hit
GGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCAT4769480.5333317648464357No Hit
GGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCAT4600300.5144137553408461No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC4005290.44787867533185605No Hit
CAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAA3433610.38395239755578603No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC3182790.35590525756174407No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA3142150.35136082023873216No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT3095630.34615887082272534No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG2958420.33081580376833375No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC2849730.31866189400853623No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT2834060.3169096466450619No Hit
GTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAG2587730.2893645864635279No Hit
GAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATT2538040.28380816199058345No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG2390410.2672999119414629No Hit
CAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCC2383400.26651604123195716No Hit
GTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCAC2219750.24821640619477925No Hit
CTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAG2216610.24786528579137726No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA2196990.24567134237903732No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT2152040.2406449531647315No Hit
GTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCC2094310.23418948154422262No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT2022490.2261584409797856No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG1963720.21958667470337287No Hit
GGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCT1941050.21705167484823798No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG1896610.21208231474403888No Hit
ATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGG1892800.2116562737450065No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG1831120.20475910607563202No Hit
CAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTAC1827250.2043263557695283No Hit
TATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGA1823760.20393609774154325No Hit
GACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTG1793240.20052329687790335No Hit
CTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCA1760510.1968633698704678No Hit
ACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGA1720130.19234800621143175No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC1623540.18154714004436173No Hit
CTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCA1580910.1767801773701491No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA1539640.1721652923228877No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG1519220.1698818914829294No Hit
CTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTC1389920.15542333474411424No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT1323480.1479938953804106No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA1310380.1465290300031602No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG1310330.14652343891393405No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA1290040.14425457490596377No Hit
CCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTG1255610.1404045508648392No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG1183630.1323556188148785No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA1179160.131855775438061No Hit
CTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG1170460.13088292591271147No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC1151520.12876502131384715No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT1151130.12872141081788321No Hit
GAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTC1150450.12864537200440762No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC1127030.12602650581088054No Hit
GGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGT1115550.12474279172455727No Hit
GGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAG1115520.12473943707102157No Hit
TCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTC1047750.11716127473390245No Hit
GGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAG1040130.11630919273583772No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT1030350.11521557568320343No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC1028150.11496956775725298No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG1020290.11409064853090273No Hit
TCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACA1016410.11365678000695376No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA1004630.1123395193852736No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC997540.111546702933006No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA951270.10637270896312993No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC948380.10604954400585867No Hit
CCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGC932020.10422013961106347No Hit
CTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGG924970.10343179603017681No Hit
GCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAG917500.1025964872997905No Hit
CTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAA916920.1025316306647672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)