FastQCFastQC Report
Thu 23 May 2024
EGAF00006692700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006692700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109364009
Total Bases13.7 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCT4183000.38248414979008316No Hit
CTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTC3366330.3078096743874852No Hit
CTCCATTTCAGATGGTATCTATGGTTCTACATTCTTCATGGCTACTGGAT2932830.2681714054575303No Hit
CTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCATTT2924400.26740058514131465No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT2818680.2577337851614419No Hit
CTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGT2760650.25242765195266387No Hit
CTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAA2465890.2254754578354932No Hit
CATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCTTCATGGCT2219320.20292964936938257No Hit
CCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTAC2212790.20233256079703515No Hit
CTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTAT2198520.2010277439628242No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA2176800.19904171581712954No Hit
CATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACT2144730.1961093068561523No Hit
CTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATC2107930.1927443972907028No Hit
CTACTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATT2099410.19196534757609335No Hit
CATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCA2065600.18887383691283666No Hit
CCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCATTTCAGAT2015510.18429371951790832No Hit
GAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTAC1896620.17342268423974838No Hit
ATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTA1858530.16993981996398833No Hit
CATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGATCA1821870.16658771168492917No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA1792310.16388481150137793No Hit
TCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGG1728400.15804102426420744No Hit
ATTTCAGATGGTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCA1675640.15321676805026416No Hit
TATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAAT1585780.1450001709428922No Hit
CAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAAT1572420.14377856247021814No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT1567750.14335154813134182No Hit
CTAATTTTAGCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCT1544240.14120184639537126No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG1541650.14096502259715077No Hit
CTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATC1531420.1400296143130598No Hit
GTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAAT1501410.13728556713753975No Hit
TTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCTT1468860.13430926805179572No Hit
GAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCTTCAT1467280.13416479639110523No Hit
CATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCC1461390.13362622798511345No Hit
CAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACA1443530.13199314959275132No Hit
CCAAGCTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCT1435940.13129913699487736No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC1431900.13092972844475737No Hit
AAACGAAACCACATAAATCAAGCCCTACTAATTACCATTATACTAGGACT1392930.1273663989402583No Hit
GATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTC1380560.12623531384991565No Hit
GGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTTTG1379740.1261603348867725No Hit
ATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGG1354120.12381769947734816No Hit
CCTAATTTTAGCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCC1345310.12301213281235877No Hit
GGTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCA1345110.12299384526037263No Hit
CTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTA1341400.12265461117102977No Hit
GCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTAC1325050.12115960379616296No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA1307590.11956310050777308No Hit
TTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGATCAACATT1279420.1169872988105255No Hit
TCTCCATTTCAGATGGTATCTATGGTTCTACATTCTTCATGGCTACTGGA1273260.11642404220935244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA1272090.11631706003023352Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTA1267700.1159156482641378No Hit
CTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGG1267060.11585712809778215No Hit
CTCCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACA1232310.11267966594019062No Hit
CTAATATTCCGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAA1195590.10932207139553562No Hit
ATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCA1192640.1090523300037401No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA1184980.10835191676267099No Hit
CTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTA1182110.10808949039166989No Hit
CAAGCTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTA1168490.10684410810141388No Hit
CTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACT1168360.10683222119262288No Hit
ATGGTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTC1159920.10606048649880785No Hit
TTCAGATGGTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATG1149430.10510130439713489No Hit
TTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCTTC1141820.10440546304406233No Hit
CAGATGGTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGA1130880.10340513395042056No Hit
CTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGATCAACAT1129250.10325609040173354No Hit
CCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACACAAATTTTGTAC1126830.10303481102270126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)