FastQCFastQC Report
Fri 26 Aug 2022
EGAF00006692706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006692706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114102194
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2831430.24814860264650127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2102230.1842409796256854Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT1598690.14011036457370837No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA1451800.12723681719915045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGAGAGTGAGTAGTAGA1401440.12282322984955049Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT1336670.11714673952719962No Hit
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC1314250.11518183427743729No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT1265850.11094002276590756No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC1215290.10650890726956574No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA1194230.10466319341764804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)