FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006692724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006692724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124569534
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2659330.21348157246859414Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTC2447700.19649266730017628Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAGATCAAAACCAACCCG2028000.16280064112626447Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA1948140.15638976380854086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG1666840.13380799835054372Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1659150.1331906724480482No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG1512980.12145666371361717Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGGGACGGCCGGGGGCA1297980.10419722690782483Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)