FastQCFastQC Report
Fri 26 Aug 2022
EGAF00006692726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006692726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58511457
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT956770.1635184029001363No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA907740.1551388474226509No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC824660.1409399188264958Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC650100.1111064453582142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATAAAATTTGAAATCTG635660.10863855261713957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT610490.10433683099021103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC607020.10374378474287524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG595040.10169632248262081Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)