FastQCFastQC Report
Sat 27 Aug 2022
EGAF00006692728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006692728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38711175
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT645020.16662372041148327No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA618490.15977040221589758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC553400.14295613605115318Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATAAAATTTGAAATCTG449310.11606725964789237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC441190.11396967413156538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT418180.1080256540908407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC405120.10465195127763495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG393220.10157790353819018Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)