FastQCFastQC Report
Fri 26 Aug 2022
EGAF00006692730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006692730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84128466
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT2485080.29539109865619084No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2377390.28259043734376427No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC1654390.19665044171850227No Hit
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC1626360.1933186324828507No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT1584450.18833696551652326No Hit
CCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGAC1274470.15149093530363433No Hit
GTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAG1247040.14823044556642695No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC1207650.14354832049356517No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA1095190.13018066916850712No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC1074010.12766309087342684No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT1072650.12750143334361996No Hit
CTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGG1067670.1269094815065331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1065660.12667056118674505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTT1052900.1251538331864984No Hit
CCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACG1034470.122963135925954No Hit
CGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGAC1016240.12079621183155771No Hit
CTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAG1003810.11931870955545534No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA1003640.11929850236422948No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC993790.11812767393143718No Hit
CAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGC991820.11789350824487872No Hit
ATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCA981700.11669058603778655No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC959200.11401610484612901No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG935130.11115500430020916No Hit
GCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATT927590.11025875593642703No Hit
CCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACT907540.10787549603008334No Hit
CCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGA904350.10749631403002165No Hit
CCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTC896930.10661432956592838No Hit
TCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACC892500.10608775393574868No Hit
CTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC885740.10528422091994404No Hit
GACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACT883560.10502509340893011No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA861480.10240053586618351No Hit
TTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACT856340.10178956549617818No Hit
CTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCC847650.10075662142704468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)