FastQCFastQC Report
Tue 30 Aug 2022
EGAF00006692732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006692732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92477083
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT2734640.2957100193136498No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2709920.29303692461839437No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT1815050.19627024784075425No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC1803580.1950299405529476No Hit
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC1765450.19090675686645522No Hit
CCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGAC1386880.1499701282749154No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC1384950.14976142792047192No Hit
GTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAG1372870.14845515834447331No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA1248950.13505508170061983No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT1227950.13278424882843676No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA1176520.1272228709895618No Hit
CTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGG1166600.126150172794702No Hit
TTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTT1160500.12549054991278216No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC1152010.12457248462302817No Hit
CCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACG1134580.12268769333911624No Hit
CGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGAC1131590.12236436999207684No Hit
CAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGC1121810.12130681068303159No Hit
CTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAG1104870.11947500549947061No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC1091690.11804978753492906No Hit
ATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCA1090000.11786703955616766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1071420.11585789313877905Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1059170.11453324063000561No Hit
GCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATT1056260.11421856807486022No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG1040200.11248192160213358No Hit
CCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACT1027370.11109455085212842No Hit
CCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTC997830.1079002459452576No Hit
CCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGA997490.10786348007970796No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA996830.10779211104658222No Hit
GACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACT982630.10625659548539176No Hit
TCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACC981910.1061787383583455No Hit
TTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACT976490.10559264720752491No Hit
CTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC956030.10338020718062657No Hit
CTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCC945170.10220586218101192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)