FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006712239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006712239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545470
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCACCA46765330.25959740402596No Hit
ACTGTGGC45387429.368023966819155No Hit
GGGTCAAG29850919.315095084343277No Hit
CTAAGTTT25174516.28921946074657No Hit
TACCCCCA91650.5930234815298906No Hit
TACCACAA44530.28813241279351914No Hit
TACAACCA41800.27046788355645857No Hit
ACTGTGGA40530.26225031867328386No Hit
TAACACCA30100.1947627582547704No Hit
CCTGTGGC27160.17573941907639748No Hit
TCCCACCA26670.17256886254666864No Hit
CTAAGTTA26160.16926889554633864No Hit
TACCAACA23590.1526396500740875No Hit
GGGTCAAT22780.14739852601473985No Hit
TACCACCC20080.12992811248358105No Hit
ACTGTTGC19920.1288928287187716No Hit
GGGTAAAG18530.11989880101198988No Hit
ACTGTGTC18360.11879881201187989No Hit
AATGTGGC18250.11808705442357341No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)