FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006712268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006712268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162955538
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13125020.8054356520242963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT5842160.35851251646323307Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATGCCCCAACTAAATACTA4480130.27492959459898814Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3859000.2368130624686103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3098220.19012670805947082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2545910.1562334138039543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATAAAATTTGAAATCTG2008130.12323177381059612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGTGATAGACAAAGTTC1774860.10891682613450057Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCC1715570.10527841036000875Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAA1675720.10283295803055187Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)