FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132808505
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA3157040.23771369160431405No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC2923790.2201508103716701No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG2788360.2099534212812651No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2467210.1857719880213997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC1908680.14371669946890825No Hit
CTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCA1900210.14307893910860603No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC1747570.13158569927430475No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT1742220.13118286362759674No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1715930.1292033217300353No Hit
GTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGT1574230.11853382432096499No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT1537550.11577195300858178No Hit
GCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAA1513290.1139452627676217No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC1440980.10850058134454565No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA1427230.10746525608431479No Hit
AAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCT1415290.10656621727652155No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAA1387840.1044993315751879No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT1373650.10343087590662962No Hit
TCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTC1341040.10097546087127478No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA1340530.10093705971616802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)