FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130980220
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTGA4376948333.416864775459985RNA PCR Primer, Index 38 (100% over 8bp)
GATACGCC3833469429.267544366622683No Hit
AGCCAATT3630776827.72003894939251No Hit
TCGTTCAG110463858.433628375337895No Hit
AGCCAATA2020550.15426375066403156No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)