FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130980220
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA3123480.23846959487470706No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC2853740.2178756456509235No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG2749470.20991490165461624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2409260.1839407507484718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC1867210.1425566394681579No Hit
CTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCA1854220.14156488666762052No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT1726100.13178325704446062No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC1710180.13056780634511075No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1694520.12937220597125276No Hit
GTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGT1536790.117329929664189No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT1514660.1156403615752058No Hit
GCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAA1486220.11346904135601543No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC1419540.10837819634140179No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA1407880.10748798559049604No Hit
AAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCT1388240.10598852254180059No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAA1372770.10480742817503283No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT1349910.10306212647986086No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA1315220.10041363497480765No Hit
TCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTC1311780.10015099989906873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)