FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123178972
Sequences flagged as poor quality0
Sequence length91
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA26015502.1120082086737986Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA25192172.045168066510573Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8479630.6883991530632355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA7598820.6168926300180521Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA5792520.4702523414467203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA3822850.31034923720584384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA2671940.21691527024596374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA2327890.18898436658490703No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA2209300.1793569116650852Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA2038760.1655120161256095No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA2024890.16438601224890886Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA1933310.15695130172055666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA1877910.1524537808287603Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAA1555660.12629265975689422Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA1394300.11319302128938045Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA1363530.1106950299926192Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA1308000.10618695535143774Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAA1298390.10540678972381747Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA1274210.10344379233819226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)