FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5473134
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAA3747366.846826699291484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2648844.839713407345773Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGAAAAAAAAAAAAAAAA2072243.7862036632028375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1139602.082170836672371Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA1031091.8839114847178966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAAAAAAAAAAAAAAAA1028601.8793619889445425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCAAAAAAAAAAAAAAA678141.239034162145491Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA392930.7179250498891494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAAAA387650.7082779263215554Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG385380.7041303940301845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGACAAAAAAAAAAAAA363260.6637147930235219Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA362770.6628195107227413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA316970.5791380222008086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAGAGAAAGAAAAAA300480.5490090321194402Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAAAAAAA299800.5477665995387652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTAAAAAAAAAAAAAAA292160.5338075040735345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAAAAAAAAAAAAAAA286590.5236305195524174Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAAAAA281710.514714238679338Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG267370.48851352808098614Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA245100.4478238610638804Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGTTAAGCACTGAAGGTG243550.44499184562263594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAAA221670.405014750232682Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAGAAAAAAAAAAAA219010.40015464631415926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAGAGAAAAAAAAAA215660.3940338387475987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAGAAAAAAAAAAAAAAAAAA209120.3820845606922834Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA193650.3538192194819275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAAAAAAAAAAAAAAAA193260.35310664785477575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACAAAAAAAAAAAAAAA191470.34983612679682247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAGAAAAAAAAAAAAAAA171620.3135680580815306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAA169900.31042543449511745Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAAAAAA168580.3080136536032189Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTGGTATCAACGCAGAGTACATGGGGGAGAAAAAAAAAAAAAAAAAAAAA158570.28972431517298863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGACAAAAAAAAAAAAAA156860.2865999626539383Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC152710.27901746969834834Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA148860.2719831087636444Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAGAAAAAAAAAAAAAAAAAAA148730.2717455848879271Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGGAGAAAAAAAAAAAA144310.2636697731135397Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAGGAAAAAAAAAAAAAA144050.26319472536210514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA128450.23469186027603198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGAGAAAAAAAAAAAAAAAAA121000.22107991509069574Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAAAAAAAAAAAAAAAA120610.22036734346354395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAAAAAAAAAAAAAAAAA118220.2160005583638186Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAGAGGAAAAAAAAAAAA111980.20459941232938936Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGGAAAAAAAAAAAAAAAAAA106610.19478784915552952Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAA105670.19307036882342002Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA105020.1918827494448336Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTAAAAAAAAAAAAAAAAA104200.1903845219210785Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAAAAAAAAA103370.1888680233299605No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAAAA102700.18764386181664838Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGCAAAAAAAAAAAA102560.18738806687356824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA101470.18539652053101568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAA99340.18150478318272492Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAGCAAAAAAAAAAAAAA98170.17936706830126944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGAAAAAAAAAAAAAAAA96120.17562149949188163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGACGAAAAAAAAAAAA95850.1751281806730842Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGAAAAAAAAAAAAAAA93370.17059695596709307Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGATAAAAAAAAAAAAA93060.1700305528788442Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGGAGAAAAAAAAAAAAAAAAAAAA88660.16199128323918252Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAGCAAAAAAAAAAAAAA84330.15407991107106092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA83420.15241724394104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGGAGAAAAAAAAAA82720.15113826922563928Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAAAAAAA80620.14730134507943712No Hit
GTGGTATCAACGCAGAGTACATGGGGAGGAAAAAAAAAAAAAAAAAAAAA80130.14640606277865661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAA78130.14275184930608315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA76440.13966403892175855Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGGAGGAAAAAAAAAAAAAAAAAAA75020.13706954735623136Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAAAAA74120.1354251512935733Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAGCAAAAAAAAAAAAAAAAA70900.12954186760272998Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCGAAAAAAAAAAAAAAA70550.12890238024502965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGTCGTACCGAAAGGAGC69820.1275685923275403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCAAAAAAAAAAAAAA68670.12546741958081056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCGGAGGAAAAAAAAAAA67190.12276330161110618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGAAAAAAAAAAAAA66570.1216304954346084Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA65560.11978511763095878Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA65510.11969376229414444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCTGTGGATAGCGGAAAA64200.11730025246960883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA64110.117135812863343Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA63470.11596646455211951No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAAA61410.11220262467536882Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGTAAAAAAAAAAAA61040.11152659518294272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAGGAAAAAAAAAAAAAAAAA60960.11138042664403977Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAGAAAAAAAAAAAAAA59880.10940715136885008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAGAAAAAAAAAAAAAAA59760.1091878985604957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAAGAAAAAAAAAAAAA59270.10829261625971517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAGGGAAAAAAAAAAAAA56470.1031767173981123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAACAAAAAAAAAAAA56340.10293919352239503Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)