FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64080995
Sequences flagged as poor quality0
Sequence length91
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCACTTTGGGAGAACGAGGCGAGCGGATCACTTGGGGTCGGGAGTTTG34515105.386167927011745No Hit
GCCTGGTTCCCTGCGCCACCTCCAACTCAGACACACAAGACACATTTACA34385505.365943521944376No Hit
NGCACTGNGNCNAGGGTTCACNTNGNCNAAGNTNTNANGTCCATCNGNAC34179725.3338310367996No Hit
AAGGCAAAGAGGAACTGCTGGCCACGAGTACGGGGTGTGGCCATGAATCC34159745.330713107685048No Hit
NTTGCAGTTGTGTAGTAGTTAAGGACTATGGCAAGGAGTCTCAGGCCAAG34139045.3274828207645655No Hit
GACAGAGGAGTGTGGCAAGTTCGGGGCCGTGAACCGCGTCATCATCTACC34081745.31854101204265No Hit
NAACACAGTGCTGTCTGGCGGCACCACCATGTACCCTGGCATTGCCGACA34079785.318235149126508No Hit
CNGGGATTTTGAACCCAGTAGTATCTGAGTTTAAGGTCTGTCCTTTTTTT34020395.308967190662379No Hit
TTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTT33980985.302817161312804No Hit
GCTACCGGCAGATGGAGGAACGCATCACCTGCCCCATCTGCATCGACAGC33949005.297826602099421No Hit
CAAGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACAGG33923735.293883155216301No Hit
NAATGTTCAGAGGACATGGGGAAATTTTAAAATTTGGGTGGTAAAGGAAG33691775.2576852154059095No Hit
CAAAGGCCCTGGCCAACGTCAACATTGGGAGCCTCATCTGCAATGTAGGG33607245.244494096884732No Hit
ANNCAGTGGTATCANCGCAGAGTACATGGGGGATCGCTTGAGACCAGGAG33521435.231103231153012Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAGCAGTGGGCTCAAGGCTGAGTCCATGGGGGGGGGGGAAGGGATGGGTG33504465.228455020088249No Hit
CNCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAG33380925.20917629322079No Hit
ANNTACTTCTGACATTGTGCACATTTGGTGTCCAGAAGGAATGAAAACCA33363565.206467221677816No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGTGGCTAACATACTATAAAAAT33050655.157636831325731Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CCACAGTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACA31275204.880573405578362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)