FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109284
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT65033030.83178936549085No Hit
TACGTTCA49122523.288708395834796No Hit
AGTCCCAC48431822.96125130613042No Hit
CTATAGTG44591321.140491275712517Clontech Universal Primer Mix Short (100% over 8bp)
CTATAGTA27120.12857443568528468No Hit
TACGTTAA23900.11330859192029144No Hit
TACCTTCA23650.11212335560313356No Hit
AGTCCCCC21230.1006502680530455No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)