FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062862
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT63270330.67112584360951No Hit
TACGTTCA48290123.40927313606048No Hit
AGTCCCAC47205522.883498750764712No Hit
CTATAGTG43693921.181203589963847Clontech Universal Primer Mix Short (100% over 8bp)
CTATAGTA27290.13229193227661376No Hit
AGTCCCCC24060.11663407440730403No Hit
TACGTTAA23330.11309530157615971No Hit
TACCTTCA23210.11251358549432779No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)