FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006717320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006717320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30732477
Sequences flagged as poor quality0
Sequence length91
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GNAGTGGTATCAACGCAGAGTACATGGGTTTAGTAATGGGGTTTGTGGGG345059311.227838875467148Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCCTCCCATCCCTACGCATCCT343838011.188099156472157Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTCCAGCCTGGCGACAGAGCGGGACTCCATCTCAAAAAAAAACCAAAAAG342796611.154213179757688No Hit
CNACTCAGGAGGCTGAAGCACAAGAATTGTGTGAACCCAGGAGGCGGAGG342638611.14907203867752No Hit
GCTAAATTTTACCATAATCACAAAGTAATTACAGTCACTACAACCAATAA342525211.145382131092134No Hit
AGCACTTTGGCAGGCCAAGGTGGGAGGATCACGAGGTCAGGAGATCGAGA340665611.08487285291062No Hit
GTTCTATTTTTTTGGTTTTCGGAACTGAGGCCATGATTAATAGGGACGGC339941411.0613082049976No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCAAAAAAAAAAAAAAA339551211.04861153886164Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCTGTGAAAGGTAAACATCGAA336231810.940602021763493Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)