FastQCFastQC Report
Thu 5 Jan 2023
EGAF00006754126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006754126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30855202
Sequences flagged as poor quality0
Sequence length41-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA1008700.32691408080880496No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG733940.23786588724974156No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT679370.22018005262127274No Hit
GGGGGCTAGTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGG674070.21846235198849126No Hit
CCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGATGC539330.17479386458076016No Hit
GTCCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGAT531570.17227889157880089No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA519960.1685161549096324No Hit
CCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCC515110.16694429678340786No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT506540.16416680726964614No Hit
GGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATAATGGCCCATTT477210.15466111678672528No Hit
GTGGCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTC472000.15297258465525523No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGGGTGAGTAGTAGAATGTTT457650.14832182916838466No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC452010.14649393641953795No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC450290.1459364939500315No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG446470.14469845311659277No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG434200.14072181410447418No Hit
GTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATAATGGCCCA415950.13480709022744367No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA411330.13330977382679265No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG408360.132347213283517No Hit
GGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGTAGAAGTAGA403870.13089202916253798No Hit
GTACGGTAGAACTGCTATTATTCATCCTATGTGGGTAATTGAGGAGTATG391950.12702882321107475No Hit
GGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAG389580.12626071934320832No Hit
GTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTGTTAGATAT385170.12483146277895052No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT371220.1203103450756861No Hit
GCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGGGACTCAGAAGTGAA349530.1132807362596427No Hit
CTGGAGTTTAAGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATA346210.11220474265571166No Hit
GTGCGAGATAGTAGTAGGGTCGTGGTGCTGGAGTTTAAGTTGAGTAGTAG346210.11220474265571166No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG338270.10963143265112962No Hit
GTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAG337190.10928141063539301No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT331090.10730443443539926No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC315630.10229393409902161No Hit
GCTAGTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCC313170.1014966617298438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)