FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006767526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006767526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37028647
Sequences flagged as poor quality0
Sequence length91
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNATCTCGGCTCGCTGTGGCCTTGACCTCCTGGGCTCAAGTGATCCTCC34198339.235641259050054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTCACGTCTGTAATCTCAGC34117939.213928340400878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNNCAGTNGNANCAACGCAGANTNCNTNGNANANGNTNGGTCCTANCNTT33989619.179274090138913No Hit
AGCGACAAATTGACGTATTTTAAATCCTATTTGGTAACTGTAAAACTATC33979109.176435747166241No Hit
GCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCT33766049.118896512745929No Hit
ANGCAGTGGTATCAACGCCAGAGTACATGGGAGATCGGAAGAGCGTCGTG33738109.111351003454162No Hit
GGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTA33730119.109193214648108No Hit
ANNATGAATAAGATATAGTCCCTGTCCTCACAAAAGTTAAGAGTATGGTG33703489.102001485498512No Hit
ANNCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC33437739.030232727650027Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ANNCAGTGGTATCAACGCAGAGTACATGGGTTTGGGAGGCCAAGGCGGGT33364599.010480453147531Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAGCTGCTCTGCCGCGGG32261458.712565166099642Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)