FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006772036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114938424
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38243923.3273398632993265Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7646310.6652527269731835No Hit
GTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAG5831860.5073899395036076No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5823580.5066695537777689Clontech SMART CDS Primer II A (100% over 24bp)
GGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCT3875960.33722056255095334No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT3685430.3206438605770338No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG3529650.3070905165708554No Hit
ATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTC3420490.2975932574123341No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA3411690.296827630070863No Hit
ATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGAT3406020.296334322454256No Hit
GGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCT3362940.29258622860532696No Hit
ATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGA2987490.2599209120876757No Hit
GGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGT2947240.25641903703151525No Hit
GTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATA2702400.2351171963172211No Hit
GTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGG2477630.21556150796012305No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2391920.20810447166040835Clontech SMART CDS Primer II A (100% over 25bp)
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT2348590.20433462703473299No Hit
GACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTG2343990.20393441274260032No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA2330810.20278771179253335No Hit
CTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCG2316580.2015496575801318No Hit
GTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGCA2305940.2006239445218076No Hit
AGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTC2295370.19970432168097244No Hit
GTGCTTTCTCGTGTTACATCGCGCCATCATTGGTATATGGTTAGTGTGTT2291910.19940329093080308No Hit
GTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGA2220540.19319387918525835No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC2205180.19185751146196334No Hit
GTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTT2191070.19062989762239996No Hit
GTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTGTTGT2096950.18244116519293843No Hit
GCTTTCTCGTGTTACATCGCGCCATCATTGGTATATGGTTAGTGTGTTGG2056000.17887838796188818No Hit
GTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGT2033580.17692777830327655No Hit
GGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGTAT1959920.17051912944273537No Hit
GAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCT1953690.16997710008621658No Hit
GGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAG1920580.16709642721393153No Hit
CTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCA1899830.16529111274398542No Hit
GATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTT1891850.1645968279502423No Hit
AGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTC1874150.1630568729566015No Hit
AGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGC1859110.1617483462275418No Hit
GCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTAT1769750.15397374858733057No Hit
CAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTT1769140.1539206766920695No Hit
GGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTGTTG1761930.15329338429070508No Hit
GTTACATCGCGCCATCATTGGTATATGGTTAGTGTGTTGGTTAGTAGGCC1684290.1465384630643622No Hit
GTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATAAGGAAAATGATTAT1593150.138608999893717No Hit
ATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGT1457410.12679919815152504No Hit
CTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGT1449730.12613101428987752No Hit
GAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTT1364080.11867919817658193No Hit
CTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTA1343490.11688780420375348No Hit
GGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGTATATG1341510.11671553805192249No Hit
TTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGA1272410.11070362335923452No Hit
GTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGTATA1264330.11000063825479284No Hit
GATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAG1253590.10906622488577014No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA1232570.107237419576938No Hit
GTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATAAGGAAAAT1199970.10440111828921544No Hit
GTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGA1166050.10144997290027225No Hit
GGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGT1165980.10144388268278326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)