FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006772061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944151
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCATA160927627.073269168296697No Hit
CTAGACCT160427626.989152866405984No Hit
TGCCGTGG127133221.38794926306549No Hit
GAGATGAC120699420.305574336856516No Hit
CTAGCCCT593430.998342740620149No Hit
GAGATGCC221630.3728539197607867Illumina PCR Primer Index 3 (100% over 8bp)
CTAGACCA88090.14819610067106304No Hit
TGCCGTGA75010.1261912760964518No Hit
TGCCGGGG70360.11836846002061523No Hit
ACTTCAAA68200.1147346357789363No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)