FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006772066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5257338
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCATA142234227.054414230167435No Hit
CTAGACCT139297426.495804530733995No Hit
TGCCGTGG111816621.268672472646806No Hit
GAGATGAC105589820.08427078494858No Hit
CTAGCCCT765661.4563644186468514No Hit
GAGATGCC301420.5733319790357781Illumina PCR Primer Index 3 (100% over 8bp)
CCTTCATA96320.18321059060688127No Hit
TGCCGGGG89730.17067572980850765No Hit
CTAGACCA77170.14678531226259373No Hit
TGCCGTGA66440.12637574376994593No Hit
CTAGACAT65350.12430245116444863No Hit
CTAGACCG62540.11895754086954273No Hit
ACTTCAAA59110.11243332652380349No Hit
ACTTAATA55790.10611834354192179No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)