FastQCFastQC Report
Wed 31 Jul 2024
EGAF00006772069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064228
Total Bases48.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCATA162494826.795628396557646No Hit
CTAGACCT158584126.150748289807048No Hit
TGCCGTGG127508621.02635323078222No Hit
GAGATGAC119612919.724340839427544No Hit
CTAGCCCT1058461.7454159045471245No Hit
GAGATGCC432930.7139078543880606Illumina PCR Primer Index 3 (100% over 8bp)
CCTTCATA167150.2756327763402036No Hit
TGCCGGGG132380.2182965416207966No Hit
CTAGACCA94700.1561616746599897No Hit
CTAGACCG90260.14884005020919397No Hit
CTAGACAT88230.14549255074182565No Hit
GAGCTGAC82640.13627455959769325No Hit
ACTTAATA77680.12809544759860614No Hit
TGCCGTGA72870.12016368777691076No Hit
ACTTCAAA72100.11889394659963314No Hit
ACGTCATA67470.11125900939080786No Hit
GAGATGAA64050.10561937974627603RNA PCR Primer, Index 45 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)