FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006772091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56508995
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT1793886931.74515667815363No Hit
AGTCCCAC1337453623.667977107007477No Hit
TACGTTCA1267759322.43464602405334No Hit
CTATAGTG1157581620.48490864153574Clontech Universal Primer Mix Short (100% over 8bp)
CTATAGTA756250.13382825159074233No Hit
TACGTTAA667990.11820949921335533No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)