FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006772117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10040536
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ANNCAGTGGTATCAACGCAGNGTANATGGGNACANCNACCTCAACCTATC336177433.48201729469423Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGTGGCCAGAAAAATAAAGGAG334009633.26611248642502Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGATTGAAAATTTCTTCATGAGGT333866633.25187021888075Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)