FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006772136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70986251
Sequences flagged as poor quality0
Sequence length91
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAAATTTAAACCTGAAAAGTAGGAAGCAGAAGAAAAAAGACAAGCTAG34679514.885384072473414No Hit
GAAATTCCTGAGGCGCTGGGAGCCGCACCACCTAACGCTGGCCGACAACA34436074.851090107575902No Hit
CNNACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTT34422974.849244679790175No Hit
TTGAAGTTGGATAAAGAGCTTAAAATAAGTAGGAACCTTAGTAAAACTTA34387984.844315556261733No Hit
GGAGAATGGAACCAGGCTGGAAAACACTCTTCAGGATATTATCCAGGAGA34380254.843226612995804No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC34348194.838710245453024Clontech SMARTer II A Oligonucleotide (100% over 24bp)
NGCAGTGGTATCAACGCAGAGTACATGGGCTTCTCCCTGTTTTGGAGAGG34092684.802715951290342Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGTCAGGTGACCCACCCGCCTCGGCCTCTCAAAGTGCTGGGATTACAGGT34072654.799894278118731No Hit
TTTGGTTTTGAGCTGGAAGAGAGTACAAGGTGCTTCCGGAAAGCTTCAAG34012044.791356004981866No Hit
GNCGGTGGCTCAGGCCTGTAATCCCAGCACTTTCGGAGGCCTGGGCAGTC34001274.789838809771768No Hit
GCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGAGAGTGG33723224.750669252838835No Hit
NCAGTGGTATCAACGCAGAGTACATGGGAGTTGAGGCTTCAGTGATACAT33720574.750295941111188Clontech SMARTer II A Oligonucleotide (95% over 23bp)
NNNGGCNNGGNCNTCATGNCNNNNNNNNNANNNNNNNNGGCAACNNNNGC33708984.748663230574045No Hit
GTAAAACTGGAGCTGAAGGTGCTGTGCTGGATGAAGCTAAAAACATCAAC33692764.746378281056144No Hit
GTTCCTTGGTTATGAAACTGAAGGGTGTCTTTTTCCAGCATAATTTTGCA33642554.739305080359857No Hit
NNGCAGTGGTATCAACGCAGAGTACATGGGACTGAAGAAGAAAGGAGCCC33586514.731410593862747Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCAGCACTTTGGGAGGCTGAGGCAGGCAAATCACAAAGTCAGGAGATCG33149644.669867690293998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGTCAATAAACAGCTTCA33043034.654849289054581Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNAAAATTCTGTCAAGATAATCAAACAATTTCATCAGAACCTGAAAGAAC33037064.6540082811247485No Hit
NGCCCACCTGGGGTCCCCTCCGAGGCTGGCCTCTCGAGCTCCTGTGTGAG33012254.650513238119872No Hit
GTTCCTTGACAGAAGTTGAGCGTCTGTGTATGCTCTACTGGGAAATTTGT32712334.6082628028912245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)