FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11968402
Sequences flagged as poor quality0
Sequence length36-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG652850.5454780011567125No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG639310.5341648784858664No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC508540.42490217156810073No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG487780.40755649751737955No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC464040.3877209338389536No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA429030.35846890838058415No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA427690.35734929358154915No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG426030.3559623080842371No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT408510.3413237623535707No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG406560.3396944721609451No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG386710.32310913353344917No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG367960.3074428816812804No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA351190.29343098602470075No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA344370.28773264801767184No Hit
CTGCTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT337340.28185884798989874No Hit
CCCTGCTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT323160.2700109839224986No Hit
CTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTG312100.26076998416329933No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG311480.2602519534353876No Hit
CTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGG302650.25287419322980625No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC288340.2409177098162311No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG273250.2283095103256057No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG269040.22479191457639874No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT261320.21834159648046583No Hit
TGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC252480.21095548094056332No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG247720.20697834180369276No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC242240.2023996185956989No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT235070.1964088438874296No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA233690.19525580775110996No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC233210.19485475170369443No Hit
CTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTGAC226680.18939871839197914No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG220970.1846278224945987No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC216950.18126897809749373No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC216110.18056713001451657No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA213880.17870389046089863No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT213880.17870389046089863No Hit
TGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAG213730.17857856044608128No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA210580.17594663013491693No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG209220.17481030466723962No Hit
CTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG206890.17286351177041012No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA205140.1714013282642077No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA188210.15725574725848948No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA185990.1554008630391927No Hit
GATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTT183500.15332038479322468No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT181430.15159083058874526No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA181430.15159083058874526No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA180420.15074694182230844No Hit
CTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGG179430.14991976372451393No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT178640.14925969231314257No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG175120.14631861463209542No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG173610.14505695914960076No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG173030.14457234975897368No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC168600.14087093665470127No Hit
CTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTG165250.13807189965711378No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT164410.13737005157413662No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT162280.13559036536373026No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG158810.1326910643542889No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG158700.13259915567675618No Hit
GGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACA158380.13233178497847917No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT157530.13162158156118084No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT156890.13108684016462682No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT151840.1268673963324427No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA151470.1265582489625599No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC150390.12565587285587498No Hit
CTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTT149650.1250375781161094No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC146980.12280670385236057No Hit
CTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGG144930.12109386031652344No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC144190.12047556557675787No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA141560.11827811265029367No Hit
CGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTA140050.11701645716779902No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA136450.1140085368121826No Hit
CTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAAATT136260.11384978546008064No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA133570.1116022005276895No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT132790.11095048445063929No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA132000.11029041303926788No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTGC131300.10970553963678693No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATTGGGAT130320.10888671687331358No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT128850.10765848272810355No Hit
CCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA127650.10665584260956475No Hit
GTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGCTGAAGATG126330.10555293847917208No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT124590.10409911030729081No Hit
CAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTT124190.10376489693444454No Hit
GTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTC123650.1033137088811021No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT123470.10316331286332127No Hit
CCTGCTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT121970.10191001271514777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)