FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31821011
Sequences flagged as poor quality0
Sequence length36-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT2127070.6684482777747067No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1608610.5055181936237035No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1301640.4090504855423984No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT1295280.40705180611640523No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG1270850.3993744887615293No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTT999310.3140409335203083No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA999190.3140032225877424No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC876800.2755412139482306No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG756710.23780199818289874No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC706940.22216138890118858No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA694420.2182268816034789No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAG673900.2117783121347087No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA665020.2089877031248316No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT662130.20807949816553598No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG647150.2033719167502252No Hit
CTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAAT640510.20128524514824497No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG619480.1946764042160697No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG604030.1898211216482091No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC593690.1865716962921134No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT577780.18157185514941684No Hit
CCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAG563450.17706854128550473No Hit
CGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAG562330.17671657258155626No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG556500.1748844497743959No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA539600.1695734934380306No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC531000.16687087660414057No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA526900.1655824197414721No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG525640.16518645494953005No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC517810.16272581659960458No Hit
AAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGC516110.16219157838825424No Hit
CAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAAC500070.1571508837352779No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG499640.15701575289358344No Hit
CATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATA495600.15574615149719787No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT495240.15563301869950016No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT489990.1539831653997417No Hit
ATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG482590.15165765789151076No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC457950.1439143464046444No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC454610.14286472544822665No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC447730.1407026319811146No Hit
CTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCT445030.1398541359983817No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAG444520.1396938645349766No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC442370.13901821032650408No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTT441140.1386316732677035No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC437960.13763233355470697No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT427130.13422892189063385No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA416700.1309512133351137No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA413890.1300681489975287No Hit
CCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGA412910.12976017638157378No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG410480.12899652999711417No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT405200.12733724896421425No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC402190.12639133307235273No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA399440.12552712420105067No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC396330.12454978253205091No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT393610.12369500139389035No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA379490.11925768166196855No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT378480.11894028131287217No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGC370190.11633508438811074No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT369540.11613081683671209No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG368480.11579770359904655No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC363250.11415413545471575No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT362850.11402843234616274No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG359360.11293167272403758No Hit
CACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTA359200.11288139148061638No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT357520.11235343842469368No Hit
CAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACT356630.11207374900816319No Hit
CGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAG352250.11069729996950757No Hit
CACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTAT350250.11006878442674244No Hit
CTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAAC344920.10839379050527338No Hit
CCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCT344520.10826808739672036No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG342830.10773699176308384No Hit
CAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA335850.10554347251883354No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTG335380.10539577136628375No Hit
GTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAA333540.10481753706693983No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA323770.1017472386405322No Hit
GCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCC320230.10063476612983792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)