FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19727609
Sequences flagged as poor quality0
Sequence length36-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1553050.787246949186797No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG1299620.6587823187290461No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1240730.6289307538485783No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC1233730.625382427236874No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC1049480.5319854017787964No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC965780.4895575535788448No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG924760.4687643596342567No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG792530.4017364699391599No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA790820.4008696644383007No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT769990.3903108582494716No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG766510.3885468330196528No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG751180.38077599774002013No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA654080.3315556385976628No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA630080.3193899473575333No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG625560.31709874217397555No Hit
TGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAG604730.3065399359851465No Hit
ACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG565680.28674534252985245No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC558740.28322743014624835No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT538890.2731653896830579No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC524180.26570883476046186No Hit
CTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGG519110.26313883248598446No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG516840.26198816085618887No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC505100.2560371102245589No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA495850.2513482500590923No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT486660.24668980412172603No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA482710.2446875341051214No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA450880.22855278609789964No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG444940.2255417775159676No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG428280.21709676018011104No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG413740.20972637890379922No Hit
GATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTT397910.2017020917233305No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG395520.20049059163733426No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG387730.1965418110223089No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC385950.1956395222553326No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT361760.18337751929288543No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA357510.18122317813577915No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG335570.1701017087270941No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC334990.16980770452212432No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA334370.16949342416508761No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT333980.1692957316824355No Hit
GGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGG331720.16815012909065666No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT330680.16762294913691772No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG329070.16680683401622567No Hit
CTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTG328150.16634048251868738No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT317110.16074426454822782No Hit
TGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGAC310180.15723142120264041No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT301540.15285177235619382No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT300420.15228404009832108No Hit
GTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTA299580.15185824090491656No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA297890.15100157348009077No Hit
CCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTC297390.15074812157925474No Hit
TGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC297030.15056563621065278No Hit
CACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTG296530.15031218430981677No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC295410.14974445205194406No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG292240.14813756700064362No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA290330.14716938073945No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT286550.14525328436912957No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT281400.14264272979051845No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC280620.14224734482521426No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA279340.141598507959074No Hit
GCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGC276390.14010314174414143No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT269700.1367119553109553No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG267860.13577925231587873No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA267290.13549031714892565No Hit
GTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATG263680.1336603944248895No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT263220.13342721867612037No Hit
CGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTA262410.13301662659676597No Hit
GTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGCTGAAGATG256710.13012727492723522No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT242350.12284813633522441No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC242180.12276196268894016No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA239840.12157580779302753No Hit
AGAAAATGTAGCCCATTTCTTGCCACCTCATGGGCTACACCTTGACCTAA236740.12000440600784412No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT236480.11987261101940938No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT232220.11771320082428641No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT231200.1171961589465809No Hit
GTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTC228440.11579710445396602No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT221560.11230960629846222No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC220720.11188380710505769No Hit
CACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATT219590.11131100580916825No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT208740.10581109956102638No Hit
TGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTA208430.10565395938250804No Hit
CTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTGAC208370.10562354515440771No Hit
CTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTG206630.10474153253949832No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT205680.10425997392790987No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC202560.10267843406669303No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT200000.10138076033441255No Hit
CAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTT198750.10074713058232246No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA198510.10062547366992117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)