FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20813073
Sequences flagged as poor quality0
Sequence length36-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT1047790.503428782477244No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG1015310.4878232061166556No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG931260.4474399335456134No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT749340.3600333309742391No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA706210.3393107783747263No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT694710.33378540497119286No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA665250.31963083971309764No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT640640.30780654062953605No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG610820.2934790071605476No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT610330.29324357820683183No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT530750.2550079942543804No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC517580.2486802405392034No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAG492120.23644754429103287No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA451300.21683487104475155No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT447910.21520608705884037No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG429760.20648560642630717No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT426960.20514029811935988No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT413810.1988221537492325No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT408240.19614595115291242No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA384490.18473485390648464No Hit
CGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTG373810.17960346364998575No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG363920.174851642522947No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC359220.1725934464362855No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG354750.17044575781769467No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT351340.1688073644867339No Hit
CATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATA350620.16846142806494743No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGG347990.16719779919092198No Hit
AAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGC345290.16590053760922283No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC323050.15521494591404164No Hit
ATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG318750.1531489367283726No Hit
CCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCA314880.15128952846127047No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT314680.15119343501077423No Hit
CTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCT293520.1410267479482727No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG284960.1369139482670339No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC284580.13673137071109107No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC274760.13201318229172598No Hit
CAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA269980.129716548824866No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT268880.1291880348471367No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT247810.11906458983735847No Hit
CAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACT247580.1189540823692878No Hit
GAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTT245670.11803638991704876No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA245000.1177144768578864No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC236100.11343831831080399No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA234990.11290499966054988No Hit
CTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAAT233010.11195367450063717No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC232700.111804729652368No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT232100.11151644930087931No Hit
AGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTC231990.11146359790310638No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA227440.10927747190431707No Hit
CACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTAT226770.10895555884515466No Hit
ATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCC225980.10857598971569454No Hit
CCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAG223980.10761505521073222No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG221730.10653400389264958No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT218930.10518869558570232No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC217870.10467940029807227No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG217220.10436709658395951No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG214590.10310346770993405Illumina Single End PCR Primer 1 (100% over 50bp)
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG209540.10067710808490413No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG209370.10059542865198234No Hit
CCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG208530.10019183615989816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)