FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27882872
Sequences flagged as poor quality0
Sequence length36-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC2112930.757787791731067No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG1478930.5304080583951323No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1322020.4741333676100511No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1232200.44192004324375195No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT1194900.42854265514685863No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG913240.3275272360752508No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT814320.2920502593850447No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA788900.28293355146485627No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA776390.2784469261272655No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG730710.26206410874747765No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG714700.25632223251607655No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA685840.24597179228882876No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC646840.23198471090065614No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT644820.2312602518133713No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT641650.23012335314669163No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG633810.2273115911445564No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG631710.22655844060827018No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT593550.21287261943461205No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT564880.20259032139874258No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC543750.19501219243125315No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC528060.18938508199585752No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA509600.18276453013878916No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT480520.1723351884267876No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA476230.17079660947408862No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT470390.1687021336969879No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT458960.16460284292091576No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGG444590.1594491413940429No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT417970.1499020617388338No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG408110.14636584064941371No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC402860.14448296430869817No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT395200.14173575806681607No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT392280.14068852017826572No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC382140.13705187901734084No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC370510.13288085961876522No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAG370100.13273381594263317No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT361970.12981804743786796No Hit
CATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATA340730.12220046772800162No Hit
AAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGC340040.12195300398036472No Hit
ATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG338130.12126799563545677No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC337960.12120702630632883No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT331470.11887943250609191No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT331210.11878618529683742No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC321950.11546515007492772No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT319930.11474069098764289No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC316810.11362172447658907No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC312110.11193610184775801No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG302040.10832456570470932No Hit
GAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTT296460.1063233371368631No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC295820.10609380554485205No Hit
GGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTC294000.10544107508007065No Hit
CAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA289160.10370524241548719No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT282050.10115528988548957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)