FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26356114
Sequences flagged as poor quality0
Sequence length36-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT1674010.6351505385050314No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT1454350.5518074477899132No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG1396840.5299870838318578No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG1301510.49381710824289193No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA1276030.4841495221943569No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1090900.4139077559005854No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA985800.3740308605434018No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT897660.34058890472244885No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT892990.33881701983835705No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT877490.33293603146503314No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG831590.31552071750789973No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC755140.28651416517624717No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAG716410.27181928261503197No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA666950.25305323842505767No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT652750.24766549423788348No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC601210.22811025935006957No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT591500.22442610469813573No Hit
CCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCA547750.207826540741173No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG546580.20738262097363824No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG534710.20287892213548628No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG524340.1989443512044302No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC514730.1952981384129694No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT496510.18838513143477828No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT486270.18449988492233718No Hit
AAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGC469620.17818256515357309No Hit
CATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATA469120.1779928558512078No Hit
CGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTG461970.1752800128273842No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC454240.17234710701281683No Hit
ATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG448180.17004783026814954No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGG446320.16934211166335067No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA432400.16406060468550104No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT427500.16220145352232124No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC419340.15910539770771975No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT417700.1584831511959616No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC409860.15550850933487387No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT406340.15417295584622223No Hit
CAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACT374610.14213400351812108No Hit
CAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA374380.14204673723903302No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC356770.1353651756097276No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG354670.13456839653979338No Hit
CTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCT352010.13355914305121006No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA348390.13218564770208538No Hit
ATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCC344070.1305465593296493No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA340450.12917306398052458No Hit
CACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTAT340140.12905544421305812No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT338310.12836110816640114No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT333530.126547487235789No Hit
CCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAG327940.12442653723534508No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG306980.11647392328019222No Hit
CTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAAT306100.11614003490802931No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG305530.11592376630333288No Hit
GAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTT305390.1158706476986706No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC304360.11547984653579811No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA304200.11541913955904122No Hit
CCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCC304000.11534325583809511No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC302980.11495624886126991No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG300620.11406082095410575No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAG299680.113704167465659No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT293070.1111962104883899No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA286810.1088210500227765No Hit
CACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCA285820.10844542560409323No Hit
CCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG282600.10722369769686077No Hit
CCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT280460.10641174188273735No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG278860.10580467211516842No Hit
CGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTA278620.10571361165003308No Hit
AGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTC273680.10383928374266403No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT271470.10300076862620945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)