FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79997826
Sequences flagged as poor quality0
Sequence length36-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG4824430.6030701384310119No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG4236830.529618142373019No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC3535920.44200201140465994No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC3477790.43473556393895No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG3426490.4283228896745269No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3206640.4008408928512632No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA2942120.3677749942854697No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG2886060.36076730385148215No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT2701200.33765917588810473No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG2668990.3336328164717876No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG2655070.33189276918600263No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG2543070.3178923887256636No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA2400340.3000506538765191No Hit
CTGCTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT2346280.29329297023646617No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA2343990.29300671245741106No Hit
CCCTGCTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT2245840.28073762904506927No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG2119630.2649609503138248No Hit
CTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTG2108660.26358966304909337No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC2062130.2577732549882043No Hit
CTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGG2022850.25286312155532825No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1857010.23213255820226913No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG1810670.22633990078680388No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG1735980.2170033970673153No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT1730760.21635087933514593No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC1681570.21020196223832383No Hit
TGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC1662010.2077568957936432No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1640710.20509432343823944No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA1625350.2031742712608215No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC1579910.19749411690262683No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG1562710.19534405847478906No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1541250.19266148557587054No Hit
CTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTGAC1536440.19206021923645775No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC1487680.1859650536003316No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA1474410.18430625852257534No Hit
CTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG1463480.18293997139372262No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT1456930.18212119914358674No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC1447740.18097241792545712No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA1397770.1747259981790005No Hit
TGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAG1395580.1744522407396421No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA1386910.1733684612879355No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1379200.17240468509731752No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG1294700.16184189805357962No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC1289720.16121938113668238No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG1268350.1585480585434909No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA1250480.15631424783968503No Hit
CTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGG1245580.1557017311945452No Hit
GATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTT1244900.15561672888460745No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1228310.15354292252891974No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1224030.15300790798989963No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG1210960.15137411359153685No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG1186100.14826652914292945No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT1177450.1471852497591622No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT1165160.14564895801043393No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT1140320.1425438736297659No Hit
CTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTG1104000.13800375025191308No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT1097080.1371387267448993No Hit
GGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACA1062820.13285611036479916No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG1062360.13279860880219418No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT1057770.13222484321011424No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT1046550.13082230509614098No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT1029090.1286397457850917No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC1007020.12588092081402313No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA1000590.12507714897152328No Hit
CTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTT984670.12308709489180368No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA977100.12214081917676113No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT964590.12057702668070004No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC963160.12039827182303679No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA961790.12022701716919157No Hit
CTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGG949130.1186444741635854No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT947510.11844196866049835No Hit
CTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAAATT931300.1164156635956582No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC914290.11428935581324423No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTGC910450.11380934276888975No Hit
CGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTA905370.11317432551229581No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA883520.11044300128856001No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT875900.1094904754036691No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT872710.10909171456734337No Hit
GTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGCTGAAGATG868950.10862170179474627No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATTGGGAT868810.10860420131917085No Hit
CAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTT864430.10805668644045402No Hit
CCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTC859110.10739166836858792No Hit
CCTGCTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT858420.10730541602468047No Hit
GTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTC849310.10616663507830824No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC846850.10585912672176866No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT838850.10485909954603018No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT831390.10392657420465401No Hit
GGCTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT808130.10101899519119432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)