FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006772946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006772946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73226932
Sequences flagged as poor quality0
Sequence length36-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC5225270.7135721594890797No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT4615590.6303131749395154No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG3708270.5064079429136811No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA2878260.39306030191187036No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT2724680.37208714411249677No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT2525370.34486901622479554No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA2267680.309678411762492No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT2250860.3073814426637457No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2206730.3013549714195318No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG2162630.29533259702864517No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1976260.26988157854271433No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1970240.26905947664173613No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG1832790.2502890603145848No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC1731800.23649768639767674No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC1720190.23491220416007597No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCAT1651150.2254839790365654No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC1604050.21905191931296536No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG1601570.21871324610458895No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA1596430.21801131856787337No Hit
CTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGAC1466910.20032383713686108No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT1430530.1953557196688235No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1336940.18257490290594175No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC1315770.17968389007476102No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1312820.1792810328309262No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC1288480.175957119164845No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT1277140.1744085086071884No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA1256120.1715379800426433No Hit
GTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAA1245390.17007267216930513No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA1212150.1655333586828409No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG1191400.16269970179824003No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC1183480.1616181325198767No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1168790.15961204000735685No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC1160780.15851818016901215No Hit
CCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAG1133660.1548146247612832No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAG1133160.15474634387249764No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT1112080.15186762160129827No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1099920.15020703038603336No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG1084330.14807803227369953No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1069440.14604462740566546No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1063080.14517609450031307No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGG1061220.14492208959403077No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1058210.1445110386435417No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAG1038180.14177570623879204No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG1021550.1395046838777842No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA1020500.1393612940113345No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC1009290.13783043648476218No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT985940.13464171897847638No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG958610.13090948559745752No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT930870.12712126188763445No Hit
CTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAAT923480.12611207035138383No Hit
AAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGC921460.12583621556069016No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG903250.12334942559111994No Hit
CATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATA899440.12282912521857396No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA891530.12174892155798633No Hit
ATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAG877160.11978652881428925No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT873490.11928534709060323No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG867950.11852879484285919No Hit
GTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAA858870.11728881390251336No Hit
CACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAA857140.11705256202731532No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT851010.11621543833080429No Hit
AATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCT848110.11581940917584803No Hit
CGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTG842920.11511065355025388No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT840570.11478973337296175No Hit
CAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACT825870.1127822752426662No Hit
CCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCA823050.11239717102991559No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA807280.11024359179761893No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC806000.1100687927223279No Hit
CTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCT804440.10985575634931695No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG792910.10828119905392185No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA780070.10652774582990858No Hit
CTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGA772930.10555269473805076No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA761730.10402320282925413No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC761110.10393853452716002No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC739020.10092188486061386No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG738760.10088637879844535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)