FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006808369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006808369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4006053
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC127120.3173198157887577No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT121950.3044143449924402No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA113250.28269720844931406No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATC103010.25713588911579555No Hit
GTGGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGA95550.23851406858571267No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG94400.23564341260587418No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTC93000.23214870097824467No Hit
GGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAAC89400.22316229965005457No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC87120.21747091214220082No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG82460.20583851486737692No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT81370.20311763224300827No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG76610.191235612709068No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCC75630.18878931456972736No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC74750.18659263868950313No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCT70020.17478550583329774No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGACTGGTAGGT60410.1507968067322125No Hit
GTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAACAG59610.14879982865928135No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC55410.13831569377639288No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATG54450.13591932008887553No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG53490.13352294640135814No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAG51600.12880508570405833No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG51300.12805621892670915No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC50840.12690795653477377No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG50690.12653352314609917No Hit
GGGCTGTTCATCTACCAGGGTGGGAGCCTCCCACTGGGGGAAGTACTTTCTGGCTGGGTTGAGGTTGTGCTTCTT49210.12283911371117656No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGG49080.12251460477432526No Hit
GGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAGCCACTGA48970.12224002028929722No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAG47470.11849568640255134No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGA43680.10903500278204009No Hit
GTCCAGAGTCCAGAAAAAGAAGGCTGAATCCAGAGTGGAAAGACAGATACAATGCCCTAGGAGGGTGCAGGGTCA43060.10748734477551845No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTTCATCTACCAGGGTGGGAGCCTCCCA42320.10564014005805715No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT41090.10256978627092553No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC40120.1001484503574965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)