FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006808649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006808649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4198244
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATC164650.3921877813676385No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA150360.35814974070111216No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC132330.31520321353403946No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC127500.3036984034277188No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT123660.2945517221009546No Hit
GTGGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGA122860.29264616349121203No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG122080.2907882438467131No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCC114340.2723519642974539No Hit
GGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAAC107870.25694075904116104No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT99650.23736114432605632No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG94780.22576105628924853No Hit
GGGCTGTTCATCTACCAGGGTGGGAGCCTCCCACTGGGGGAAGTACTTTCTGGCTGGGTTGAGGTTGTGCTTCTT87850.2092541548323537No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTC87630.20873012621467452No Hit
GGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAGCCACTGA83830.1996787228183974No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGACTGGTAGGT82240.19589142508153407No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG80010.19057968045687673No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC79880.19027002718279357No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGG77340.18421987859686098No Hit
GTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAACAG74240.1768358389841086No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGA70410.16771297713996614No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTTCATCTACCAGGGTGGGAGCCTCCCA67780.16144845321043752No Hit
GTCCAGAGTCCAGAAAAAGAAGGCTGAATCCAGAGTGGAAAGACAGATACAATGCCCTAGGAGGGTGCAGGGTCA66460.15830428150436232No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG66390.1581375451260098No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCT65020.1548742760068257No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAACAATGG62070.14784752863340006No Hit
GTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGCAGTGGCAAAGCCTACTCTGTTGTTG59180.1409636981557051No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC57470.13689056662738042No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT54220.1291492347753013No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATG52960.12614797996495677No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACC52160.12424242135521424No Hit
GTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAACAGTCAGG51800.12338491998083008No Hit
GCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGACTGGTAGGTGGAAGAATCCTCAGG51790.1233611004982083No Hit
GGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTGGAGGAGCCGGTGTCAAAGACGACGG48710.11602469985069948No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG48240.11490518416747574No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC48100.1145717114107708No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTTC47400.11290434762724606No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG46010.10959343954281837No Hit
GCCAGGTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCC45740.10895031351203027No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT45140.10752114455472336No Hit
GGCTGTTCATCTACCAGGGTGGGAGCCTCCCACTGGGGGAAGTACTTTCTGGCTGGGTTGAGGTTGTGCTTCTTC44950.10706857438490949No Hit
GGGTAGAATATTTTGTCACCAAATATAAACTTGAAGAGGGATTGAGGAACAAATCTAAGTTTGTTTATAAGGCTT44540.10609197559741644No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGA44410.10578232232333327No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG44390.10573468335808972No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCCTGGGAGGTGGCTGAAGAGACTTAGG43970.10473426508797487No Hit
GTTGTGGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTGGAGGAGCCGGTGTCAAAGAC43640.10394822216145606No Hit
GTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTGGAGGAGCCGGTGTCAAAGACGACGGTGAAA43630.1039244026788343No Hit
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCT43330.10320981820018084No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCG42930.10225703889530956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)