FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006808659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006808659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4034575
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATC151890.37647087983244826No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA139150.3448938240087246No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC125220.310367262970697No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC117400.2909848001338431No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG115180.2854823618348897No Hit
GTGGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGA114820.2845900745431675No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT114440.2836482157352385No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCC107050.2653315404968305No Hit
GGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAAC100710.24961736985927885No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT93020.23055712187776903No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG86770.21506602306314793No Hit
GGGCTGTTCATCTACCAGGGTGGGAGCCTCCCACTGGGGGAAGTACTTTCTGGCTGGGTTGAGGTTGTGCTTCTT81060.20091335518611003No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTC79820.19783992118128924No Hit
GGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAGCCACTGA77070.19102383770285594No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGACTGGTAGGT75640.18747947429407064No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC74520.18470346938649052No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG74230.1839846824014921No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGG73100.1811838917358086No Hit
GTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAACAG69890.1732276633846192No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGA67440.1671551526492877No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCT63350.15701777758499966No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG63090.15637334787431142No Hit
GTCCAGAGTCCAGAAAAAGAAGGCTGAATCCAGAGTGGAAAGACAGATACAATGCCCTAGGAGGGTGCAGGGTCA60990.1511683386725987No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTTCATCTACCAGGGTGGGAGCCTCCCA60650.15032562289708334No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAACAATGG58350.14462489853330276No Hit
GTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGCAGTGGCAAAGCCTACTCTGTTGTTG54740.13567723985797758No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC54700.13557809682556404No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT51400.12739879665144407No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATG50150.12430057688851985No Hit
GTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAACAGTCAGG48530.12028528407577006No Hit
GCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGACTGGTAGGTGGAAGAATCCTCAGG48350.11983914042990897No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACC47500.11773235099112049No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTTC46570.11542727548750488No Hit
GGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTGGAGGAGCCGGTGTCAAAGACGACGG44900.11128805388423813No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT44730.11086669599648041No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG44320.10985047991424128No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG44040.10915647868734626No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGA43330.10739668986200529No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC43050.10670268863511027No Hit
GGGTAGAATATTTTGTCACCAAATATAAACTTGAAGAGGGATTGAGGAACAAATCTAAGTTTGTTTATAAGGCTT42270.10476939950304555No Hit
GTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTGGAGGAGCCGGTGTCAAAGACGACGGTGAAA41900.10385232645321997No Hit
GCCAGGTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCC41710.1033813970492555No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG41620.10315832522632495No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCG40610.10065496365788218No Hit
GGCTGTTCATCTACCAGGGTGGGAGCCTCCCACTGGGGGAAGTACTTTCTGGCTGGGTTGAGGTTGTGCTTCTTC40370.10006010546340072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)