FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006809509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006809509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4045570
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCG162360.40132787221578176No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCC128560.31777969482668694No Hit
CCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCC124840.3085844516347511No Hit
CCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCC115230.28483007338891675No Hit
CCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGC113430.2803807621670123No Hit
CCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCT105300.2602847064814105No Hit
CCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACT101260.25029847462780275No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT89950.22234196911683643No Hit
CCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGA87170.21547025511856177No Hit
CCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGC79350.19614046969895468No Hit
CCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACTCTTTGAATGAATGGCTGCTTCCAAG75000.18538796757935225No Hit
CTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCGGAGTTCGTCA71560.17688483946637926No Hit
CCCGACTTACCCCGGGATGATTGGCATTGCCCGGGAAACCTTAGACTTACGGCGGAGGGGAATCTTACCCCTCTT69850.17265799380557004No Hit
CTGGGAAGTCCTACATCCTTTTCCACTTAGTACAGCATTGGGGACCTTAGTCGGCGGTCTGGGCTGTTTCCCTCT68270.168752487288565No Hit
GTCCCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCA63720.15750561725541765No Hit
GTCGTTACTTATACCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTA61910.15303158763783598No Hit
CCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCC61190.15125186314907416No Hit
CTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGTTTCGCTACTCATACCGGCATTCTCTCTGCCATACGGTCC60150.1486811499986405No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCTA58720.14514641941679418No Hit
CTCCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGC58010.14339141332370964No Hit
CGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCCA56300.13916456766290042No Hit
GCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGC56300.13916456766290042No Hit
CCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCAC56250.13904097568451418No Hit
CTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCGA55690.13765674552658833No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCCT55390.13691519365627094No Hit
CTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCG55230.13651969932543498No Hit
CCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCAC55090.13617364178595354No Hit
CTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATT54710.13523434275021814No Hit
CCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGT53980.1334298998657791No Hit
CGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCCA53470.13216926168623952No Hit
ACCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGG53010.13103221548508615No Hit
CCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTC52310.12930192778767888No Hit
GCCTGTTGTCCATCGCCTATGCTTTTTGCCTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAG51140.12640987549344096No Hit
GTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCC50710.12534698447931936No Hit
CTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCA49040.1212190124012191No Hit
CCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTG48320.11943928791245734No Hit
CTTTATTTGAAACCTCCGGGCCGGCAGATGGCTAGTGCTTCCAGAGGGGCTGCTGAGCTAGGCTCAGGCAGCGTA45910.11348215455424082No Hit
GCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCA43810.10829129146201896No Hit
CGCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACG42100.10406444580120973No Hit
CCCCGCGGCAGTGTCACTGCTCCGTCTCCACGTCGCTCCTGAAGGTAGTAACGGAATGTTGACCGTTTCTGCCAT41980.10376782505308277No Hit
CGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGCG41590.10280380762167012No Hit
CTGCGCACATGGTATGCTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTAT41120.10164204302483952No Hit
CTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCC41050.10146901425509879No Hit
GCCTAGTTCCTTAACCATGAATCTCTCAACGCCTCAGTATGTTCTACCCGTCTACCTGTGTCGGATTGCGGTACG40950.1012218302983263No Hit
CTTTTCCTCCGGTTACTTAGATGTTTCAGTTCACCGGGTTTGTCTTCCTTTCGGATGGTACAACATGACTTGTAC40880.10104880152858559No Hit
CCTCACGGTACTATGCGCTATCGGTCGTTTCGTCGTATTTTGCCTTGGATGGTGGTCCACCCTGCTTCCCACAAG40600.10035668644962267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)