FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006809513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006809513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3965477
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCG156440.3945048729320584No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCC124800.31471623716390235No Hit
CCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCC121670.3068231135875961No Hit
CCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGC113410.2859933369932545No Hit
CCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCC110720.27920978989412876No Hit
CCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCT103770.2616835250841198No Hit
CCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACT97340.24546857792896037No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT86280.2175778601161979No Hit
CCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGA83790.211298665961245No Hit
CCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGC78990.19919419530109495No Hit
CCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACTCTTTGAATGAATGGCTGCTTCCAAG73700.18585405992772117No Hit
CTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCGGAGTTCGTCA69370.17493481868637745No Hit
CCCGACTTACCCCGGGATGATTGGCATTGCCCGGGAAACCTTAGACTTACGGCGGAGGGGAATCTTACCCCTCTT68520.17279131867364253No Hit
CTGGGAAGTCCTACATCCTTTTCCACTTAGTACAGCATTGGGGACCTTAGTCGGCGGTCTGGGCTGTTTCCCTCT66850.16857997158979865No Hit
GTCCCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCA62290.15708072446265609No Hit
GTCGTTACTTATACCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTA61970.15627375975197938No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCTA59050.14891020676705474No Hit
CCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCC58880.14848150676450778No Hit
CTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGTTTCGCTACTCATACCGGCATTCTCTCTGCCATACGGTCC57880.14595974204364318No Hit
CTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCGA56300.1419753537846771No Hit
CTCCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGC56060.1413701302516696No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCCT54380.13713356552061706No Hit
GCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGC54290.13690660669573926No Hit
CGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCCA54160.13657877728202686No Hit
CCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCAC53020.13370396550024122No Hit
CTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATT52900.13340135373373746No Hit
CCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGT52520.1324430831398089No Hit
CTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCG52430.1322161243149311No Hit
CCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCAC52420.13219090666772246No Hit
CGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCCA52120.13143437725146306No Hit
CCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTC51660.13027436547986534No Hit
GTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCC51630.1301987125382394No Hit
ACCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGG50820.12815608311433907No Hit
GCCTGTTGTCCATCGCCTATGCTTTTTGCCTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAG50570.12752564193412294No Hit
CCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTG48440.12215428307868131No Hit
CTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCA47670.12021252424361559No Hit
CTTTATTTGAAACCTCCGGGCCGGCAGATGGCTAGTGCTTCCAGAGGGGCTGCTGAGCTAGGCTCAGGCAGCGTA45020.11352984773332439No Hit
CTTTTCCTCCGGTTACTTAGATGTTTCAGTTCACCGGGTTTGTCTTCCTTTCGGATGGTACAACATGACTTGTAC41870.10558628886260091No Hit
GCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCA41550.1047793241519242No Hit
CGCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACG41480.1046028006214637No Hit
CCCCGCGGCAGTGTCACTGCTCCGTCTCCACGTCGCTCCTGAAGGTAGTAACGGAATGTTGACCGTTTCTGCCAT41470.10457758297425505No Hit
GCCTAGTTCCTTAACCATGAATCTCTCAACGCCTCAGTATGTTCTACCCGTCTACCTGTGTCGGATTGCGGTACG41070.10356887708590921No Hit
CTGCGCACATGGTATGCTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTAT40780.10283756531685848No Hit
CTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCC40150.10124885354271378No Hit
CGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGC39690.10008884177111607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)