FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006809515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006809515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998788
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCG159910.39989616853906734No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCC125400.3135950192908451No Hit
CCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCC122030.30516746574211984No Hit
CCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCC111420.27863442623114804No Hit
CCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGC110140.2754334563372702No Hit
CCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCT104940.26242951614339144No Hit
CCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACT100360.2509760457418598No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT86920.2173658618561424No Hit
CCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGA84640.2116641342326725No Hit
CCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGC80210.20058577749057965No Hit
CCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACTCTTTGAATGAATGGCTGCTTCCAAG73730.18438086740282306No Hit
CTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCGGAGTTCGTCA69850.17467792741200583No Hit
CCCGACTTACCCCGGGATGATTGGCATTGCCCGGGAAACCTTAGACTTACGGCGGAGGGGAATCTTACCCCTCTT69280.17325249550613836No Hit
CTGGGAAGTCCTACATCCTTTTCCACTTAGTACAGCATTGGGGACCTTAGTCGGCGGTCTGGGCTGTTTCCCTCT65960.1649499798438927No Hit
GTCCCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCA61990.1550219716574122No Hit
GTCGTTACTTATACCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTA60800.15204606995919764No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCTA60110.1503205471257791No Hit
CCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCC59950.1499204258890444No Hit
CTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGTTTCGCTACTCATACCGGCATTCTCTCTGCCATACGGTCC59060.14769475150970743No Hit
CTCCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGC56320.14084267533062517No Hit
CCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCAC55910.13981736466149244No Hit
GCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGC55880.13974234192960466No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCCT55420.13859199337399233No Hit
CTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCGA54860.13719156904542076No Hit
CGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCCA54670.13671642507679826No Hit
CTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATT54460.13619126595358394No Hit
CCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGT53960.1349408870887879No Hit
CTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCG53470.1337155158012878No Hit
CCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCAC53050.13266519755485912No Hit
CGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCCA52780.13198999296786926No Hit
GCCTGTTGTCCATCGCCTATGCTTTTTGCCTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAG51350.1284139094145526No Hit
CCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTC51200.1280387957551138No Hit
ACCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGG50520.1263382804989912No Hit
GTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCC48680.12173688627654178No Hit
CCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTG48530.12136177261710297No Hit
CTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCA46780.11698544659031686No Hit
CTTTATTTGAAACCTCCGGGCCGGCAGATGGCTAGTGCTTCCAGAGGGGCTGCTGAGCTAGGCTCAGGCAGCGTA45440.1136344312326635No Hit
GCCTAGTTCCTTAACCATGAATCTCTCAACGCCTCAGTATGTTCTACCCGTCTACCTGTGTCGGATTGCGGTACG42500.10628220350766282No Hit
CGCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACG42080.10523188526123416No Hit
CCCCGCGGCAGTGTCACTGCTCCGTCTCCACGTCGCTCCTGAAGGTAGTAACGGAATGTTGACCGTTTCTGCCAT41320.10333130938674419No Hit
CTGCGCACATGGTATGCTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTAT41090.102756135108938No Hit
GTTACTTATACCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGC40020.10008032433827449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)