FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006809526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006809526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3946874
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGTGTAGGAGTGAAAGGCCAATCAAACTTGGAGATAGCTCGTACTCCCCGAAAGGCATTTAGGTGCCGCGT134830.34161212139024455No Hit
GTTTCGACGGGGTATAGAAGCCACGAAGCTCGCAGGCCAATGGTTTTGAAGCCGAAAGCTAAACCGCAAAATTCA103810.2630182772492864No Hit
GTTTGATCCTGGCTCAGGATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACGGGGAAGCT92060.2332478817413477No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACAATTAAAAGCTTG91160.23096759612797368No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACAATTAAAAGCTTGCTTTTAAACGTCGAGTG56960.14431674281976065No Hit
GAAGAATCCAGACCGCCGTCTAAGGCCCCGAAATTCTGTCTGAGTTAGTCTAACGAAGTCCGGTCCCCATGACAG56400.14289789843810569No Hit
GGGAAACAGCCCAGACCGCCGACTAAGGTCCCCAATGCTGTACTAAGTGGAAAAGGATGTAGGACTTCCCAGACA56120.1421884762472782No Hit
GGTGGATGCCTTGGCGTTTCGAGTCGAAGAAGGACGCGATAAGCTGCGAAAAGCCATGGGGAGCCGCAAGTAGGC55630.14094698741333014No Hit
CGGGTGAGTAACGCGTAGACAACCTGCCGCAAAGATGGGGACAACAGTTCGAAAGGACTGCTAATACCGAATGTT50000.12668253407633484No Hit
CGAGAGGAGAAGAATCCAGACCGCCGTCTAAGGCCCCGAAATTCTGTCTGAGTTAGTCTAACGAAGTCCGGTCCC46260.11720668052742499No Hit
CGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACAATTAAAAGCTTGCTTTTAAACGTCGAGTGGC45690.11576249963895478No Hit
CGAGACCTGACGAACTCCGAATGCTCACGGACTGCAGCCATGCAGTAAGGGGGCGGGTGCTAAGGTCCGTCCCCG42930.1087696237579411No Hit
GGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACAATTAAAAGCTTGCTTTTAAACGTCGAGT42270.10709741430813347No Hit
CCCGTATTCAAGAGGGAAACAGCCCAGACCGCCGACTAAGGTCCCCAATGCTGTACTAAGTGGAAAAGGATGTAG40370.10228347801323275No Hit
GTGAAAGGCCAATCAAACTTGGAGATAGCTCGTACTCCCCGAAAGGCATTTAGGTGCCGCGTGTGGCGATCTCCG39840.1009406431520236No Hit
CAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACAATTAAAAGCTTGCTTTTAAACGTCGA39650.10045924952253353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)