FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006809527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006809527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3970766
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCG158740.3997717317011378No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCC122460.3084039704177985No Hit
CCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCC119570.3011257777466615No Hit
CCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGC108310.2727685287926813No Hit
CCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCC106060.2671021158134224No Hit
CCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCT103700.26115867819962196No Hit
CCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACT98220.24735781458791578No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT84300.21230160628956732No Hit
CCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGA82810.2085491816944136No Hit
CCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGC76180.191852151448864No Hit
CCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACTCTTTGAATGAATGGCTGCTTCCAAG72140.18167779214388358No Hit
CTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCGGAGTTCGTCA67830.17082346328139206No Hit
CCCGACTTACCCCGGGATGATTGGCATTGCCCGGGAAACCTTAGACTTACGGCGGAGGGGAATCTTACCCCTCTT65640.16530815464824672No Hit
CTGGGAAGTCCTACATCCTTTTCCACTTAGTACAGCATTGGGGACCTTAGTCGGCGGTCTGGGCTGTTTCCCTCT64360.16208459526449054No Hit
GTCCCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCA60160.15150729103654056No Hit
GTCGTTACTTATACCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTA59860.1507517693059727No Hit
CTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGTTTCGCTACTCATACCGGCATTCTCTCTGCCATACGGTCC58000.146067534576452No Hit
CCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCC57340.14440538676920273No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCTA57310.14432983459614593No Hit
CTCCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGC55700.1402752013087651No Hit
GCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGC53800.13549023034850205No Hit
CTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCGA53760.13538949411775963No Hit
CTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCG53540.13483544484867654No Hit
CCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGT52830.1330473767529993No Hit
CTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATT52570.13239259125317382No Hit
CCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCAC52460.13211556661863227No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCCT52210.1314859651764924No Hit
CCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCAC51970.13088154779203812No Hit
CGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCCA51880.13065489127286775No Hit
CGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCCA50450.12705357102382767No Hit
ACCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGG50120.126222497120203No Hit
GTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCC49860.12556771162037753No Hit
CCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTC49420.12445961308221135No Hit
GCCTGTTGTCCATCGCCTATGCTTTTTGCCTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAG48810.12292338556339005No Hit
CCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTG48230.12146271021762552No Hit
CTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCA46590.1173325247571879No Hit
CTTTATTTGAAACCTCCGGGCCGGCAGATGGCTAGTGCTTCCAGAGGGGCTGCTGAGCTAGGCTCAGGCAGCGTA43700.1100543320860509No Hit
GCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCA41310.10403534229919366No Hit
CCCCGCGGCAGTGTCACTGCTCCGTCTCCACGTCGCTCCTGAAGGTAGTAACGGAATGTTGACCGTTTCTGCCAT41300.10401015824150807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)