FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006809531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006809531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3929909
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCG154360.3927826318624681No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCC122720.3122718617657559No Hit
CCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCC120250.3059867289547926No Hit
CCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGC110580.28138056122928035No Hit
CCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCC108660.27649495191873397No Hit
CCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCT103250.26272872985099655No Hit
CCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACT100240.25506951942144207No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT83920.21354184028179787No Hit
CCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGA83340.21206597913590364No Hit
CCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGC76410.19443198303065035No Hit
CCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCACTCTTTGAATGAATGGCTGCTTCCAAG71090.18089477389934475No Hit
CTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCGGAGTTCGTCA68040.17313377994248721No Hit
CCCGACTTACCCCGGGATGATTGGCATTGCCCGGGAAACCTTAGACTTACGGCGGAGGGGAATCTTACCCCTCTT67500.17175970232389604No Hit
CTGGGAAGTCCTACATCCTTTTCCACTTAGTACAGCATTGGGGACCTTAGTCGGCGGTCTGGGCTGTTTCCCTCT63760.16224294252106092No Hit
GTCCCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCA60720.15450739444602915No Hit
GTCGTTACTTATACCTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTA60400.15369312622760475No Hit
CTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGTTTCGCTACTCATACCGGCATTCTCTCTGCCATACGGTCC59260.15079229569946784No Hit
CCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCC59030.15020704041747532No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCTA56150.14287862645165575No Hit
CTCCCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGC55260.14061394296916288No Hit
GCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGC54510.13870550183223074No Hit
CGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCACTCTTTCAATGGTGGCTGCTTCTGAGCCA54210.13794212537745784No Hit
CTACATTTGCTTTACCATGCGCTCCAGGATCAGTCATCTTCACCATTCTGCGCACATGGTATGCTCCCCTACCGA53370.1358046713040938No Hit
CCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCATGCCCGGACCCTCGACTAGTGAGCTGTTACGCAC52770.13427791839454806No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCATCTTCATGCAGGCGAGTTGCAGCCT52590.13381989252168436No Hit
CCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCCGT52110.13259849019404774No Hit
CTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGCTGCAGTCCGTGAGCATTCG52010.13234403137579012No Hit
CGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACGCTCCCCTACCCA51540.1311480749299793No Hit
CTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATT51450.13091906199354741No Hit
CCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCAC51310.13056281964798677No Hit
ACCGGACTTCGTTAGACTAACTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGG50270.12791644793810747No Hit
CCCTACCCAAGCATTGCTGCTTGCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTC50160.1276365432380241No Hit
GCCTGTTGTCCATCGCCTATGCTTTTTGCCTCGGCTTAGGTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAG49790.1266950456104709No Hit
GTCCCGACTTACCCTGAGTCGACGAGCGTTGCTCAGGATCCCTTAGGCTTTCGGTGGGACGGATTCTCACCGTCC48210.12267459628200043No Hit
CCCGGCAACAGAGCTTTACGATCCGAAGACCTTCTTCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTG47160.12000277869029538No Hit
CTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTATACCCGATTATTATCCA45570.11595688347999916No Hit
CTTTATTTGAAACCTCCGGGCCGGCAGATGGCTAGTGCTTCCAGAGGGGCTGCTGAGCTAGGCTCAGGCAGCGTA43270.11010433066007382No Hit
GCCTAGTTCCTTAACCATGAATCTCTCAACGCCTCAGTATGTTCTACCCGTCTACCTGTGTCGGATTGCGGTACG41640.10595665192247455No Hit
GCCGCGACTTCGGTTCTGTACTTGAGCCCCGGATATTTTCGGCGCAGGGCCTCTCGACCAGTGAGCTATTACGCA41330.10516782958587591No Hit
CCCCGCGGCAGTGTCACTGCTCCGTCTCCACGTCGCTCCTGAAGGTAGTAACGGAATGTTGACCGTTTCTGCCAT41080.10453168254023185No Hit
CGCTACTCATACCGGCATTCTCTCTGCCATACGGTCCAGCTGTCCTTCCGGTCAGCCTTCGTCCTATATGGTACG40450.10292859198520882No Hit
CTGCGCACATGGTATGCTCCCCTACCGATACTTTTAATATACATTGCTATCCCGCGCCTTCGGCATCTGACTTAT40070.10196164847582985No Hit
CGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTGCATGGC39530.10058757085723868No Hit
CTCAGACAGAATTTCGGGGCCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCC39520.10056212497541292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)