FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006810879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006810879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916886
Sequences flagged as poor quality0
Sequence length75
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCG495031.2638356081846651No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATG308740.7882281996463517No Hit
GTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCG292390.7464858563665115No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTC267720.6835021494115479No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTC255080.6512316161358793No Hit
CCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGA202950.5181411968589333No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCC194270.49598073571709766No Hit
GGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTC178350.45533620330027474No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174530.44558355795905213No Hit
GGGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCC171930.43894563181057605No Hit
CTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCG153910.39293969750459934No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGA151810.38757829561544554No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAG150900.3852550214634789No Hit
CCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGAC145470.37139196800723845No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGG144820.36973248647011936No Hit
GCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCG143550.3664901148514406No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGC138530.3536738112878445No Hit
CGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTG137940.3521675126618441No Hit
GGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGC121280.3096337243412241No Hit
GGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGG118340.30212776169640887No Hit
GCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGG113410.2895412324994907No Hit
GGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCC113330.28933698861799906No Hit
GTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGG111600.28492021468074386No Hit
CCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGGG108430.2768270508766403No Hit
GGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACC107460.274350593813555No Hit
GGGGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGC103230.26355119857968806No Hit
GTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTC97920.24999451094568492No Hit
GGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCC97740.24953496221232888No Hit
GACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC94010.2400120912377843No Hit
GGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGC92650.23653994525242755No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGG87580.22359598926289917No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG85120.2173154899070333No Hit
GGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGG83660.21358803906981208No Hit
GGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG82360.210269075995574No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTCCTC81400.20781814941767518No Hit
GCCCGCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCA81390.20779261893248874No Hit
GGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCC80640.20587783254350525No Hit
GGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCC80580.2057246496323865No Hit
GTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCC79020.2017418939433009No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTC78000.19913778445428332No Hit
GGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATC77190.19706981515418115No Hit
GGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCC76590.19553798604299436No Hit
GGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGG74250.18956385250936586No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGT73870.18859369407228088No Hit
GACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGG72600.18535132245360217No Hit
GGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCAC70810.18078136560522823No Hit
CTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGA69950.17858574387919382No Hit
GGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGA69550.17756452447173596No Hit
GGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGG69200.17667095749021033No Hit
CGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGC68690.17536890274570155No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCC68320.17442427479380304No Hit
GGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCG67670.172764793256684No Hit
GGCGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGAC64680.16513117818593648No Hit
CCCGACCCTTCTCCCCCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAG64330.16423761120441085No Hit
GGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACG63120.1611484224968508No Hit
CGGGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGC62930.16066334327830833No Hit
GCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCC62900.160586751822749No Hit
GGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGC61130.15606785594474795No Hit
GACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCT60560.15461261828912048No Hit
GGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAG59210.1511660027889502No Hit
CGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTC58880.15032349677779747No Hit
GAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCC58130.14840871038881398No Hit
GGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGC57660.147208777585051No Hit
GGGGCGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCG56920.14531952168125392No Hit
CGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGC55590.14192396715145655No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTCCTCCTCGCT55390.1414133574477276No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATT55270.14110699162549026No Hit
CGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACAC54810.13993258930691369No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCG54060.1380178029179302No Hit
GGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCG53190.13579665070670935No Hit
GACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCC51910.1325287486028442No Hit
GGCTGAGTGAAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGATTTTC50540.12903107213230103No Hit
GGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGC49670.12680991992108018No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA49020.12515043838396114TruSeq Adapter, Index 7 (97% over 36bp)
GGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCG48750.12446111528392709No Hit
GGGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCG48650.12420581043206262No Hit
GGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCG48430.12364413975796079No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTC47750.12190806676528243No Hit
GCGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACA47190.12047835959484142No Hit
CACCGTTCGGCCTCGGGCACCTGAGGGACAACCCGGAGCGCTCCAGGAGCACCGCAAGGGCCCAGGCGGAGCCGA46680.11917630485033263No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTCCTCCTCGC44990.11486165285382317No Hit
GGGAGGAGGAGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGA44980.11483612236863673No Hit
GGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGC44960.11478506139826383No Hit
GGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGA44160.11274262258334809No Hit
GGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTC44090.11256390918704298No Hit
GGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGC43830.11190011657219537No Hit
GCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCC43560.1112107934721613No Hit
GACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCA43460.11095548862029685No Hit
GACGGGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGC43020.10983214727209319No Hit
GGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGG42900.10952578144985584No Hit
GGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGG42550.1086322144683302No Hit
CGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGC42410.10827478767571994No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA42090.10745781214975365No Hit
GGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCC41820.10676848904971958No Hit
GTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCG41660.10636000128673645No Hit
GACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACC41460.10584939158300752No Hit
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCCGGGCCCGACGGCGC41390.1056706781867024No Hit
GTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACCGTGGGACGCTTTCCCAGGGCCAGGCG41360.10559408673114305No Hit
GCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGC41120.10498135508666834No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG40770.10408778810514271No Hit
CGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACG40000.10212194074578633No Hit
GCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCA39720.10140708716056583No Hit
CCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGGGT39670.10127943473463358No Hit
GGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCC39410.10061564211978598No Hit
GGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCC39280.10028374581236216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)